From 2007.igem.org

Saint-Petersburg iGem2007 team

This year our team is participating for the first time in iGem2007 project.

Our team works at the [http://www.spbstu.ru Saint-Petersburg State Polytechnical University] in the department of Biophysics.

About Us

• Students:
  • Tatiana Moiseeva
  • Alexey Shalygin
  • Maria Ditina
  • Gennady Zakharov

• Our Instructors:
  • Evgeny Zatulovskiy
  • Vasiliy Romanov
  • Alexej Skvortsov

Our Project

General

The aim of our project is to build on the E.Coli bacteria the copper sensor. To solve this problem we need to use the copper-homeostasis system of E.Coli. The most convenient for our purposes protein is CusR, the component of the Copper-Responsive Two-Component System.

We intend to use the CusR promoter with the reporter gene to build sensor.

Problem

The main problem of our project is to make the "trigger" response with the two stable levels (high copper сoncentration/high copper сoncentration). Constructing such switch is a challenge.

So we perform a theoretical study of this problem.