From 2007.igem.org

Making Electrophoresis Gel

1. Take 60ml TAE 1X and add 0.9g agarose to make a 1.5% agarose gel.
2. Microwave for about 1-1.5 minutes and shake after every 20 seconds. Make SURE that all the agarose is COMPLETELY dissolved (the solution should be clear).
3. Let the solution cool by placing the flask under running tap water for about a minute to a minute and a half. No water should get inside the flask. Make sure that you don't cool the gel flask too much otherwise it will solidify.
4. Add 1ul of Etbr and swirl around.
5. Pour it on the gel-plate with a fork already in it.

Loading

Load 10ul of diluted ladder in a lane.

The Fisher 1/40loading dye should have a final volume 1/5 in the overall solution.

Back