BerkiGEM2007-AustinConstructionFile39

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PCR AD0010F/AD0010R on I716063 (462 bp)
Digest PCR product (EcoR1/Nco1/DpnI, 446/11/5, Largest)

Digest petDUET (EcoR1/Nco1, 5377/43, Larger)

Ligation product is I716089

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