From 2007.igem.org

AustinDay 02:21, 31 July 2007 (EDT)

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Enzyme-Catalyzed P-F Bond Hydrolysis of Monofluorophosphate as a Simple Model of Sarin Detoxification Norimasa Yoza1), Sachiko Nakashima1) and Tetsuya Nakazato1) 1) Department of Chemistry, Faculty of Science, Kyushu University (Received September 17, 1996)

The catalytic ability of alkaline phosphatase (EC3.1.3.1) to promote P-F bond hydrolysis was examined with a simple nontoxic P-F compound, monofluorophosphate (Na2PO3F), to obtain an insight into the enzymatic degradation of Sarin, a toxic P-F compound. The enzyme recognized inorganic monofluorophosphate as a substrate and accelerated its hydrolytic conversion to orthophosphate and fluoride ions at pH 7.2 - 9.0 and 30 °C by a factor of ten billion (1010).