From 2007.igem.org
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construct Ptet-wbbL-neuS the old fashioned way
[dig] pSB1A2-Bca9296 XbaI/AlwNI, 2698+1525, L
[dig] pSB1A2-r0040 SpeI/AlwNI, 1587+ 546, L
[ligate] get I716023