NYMU Taipei/Lab Notes/2007 8 6&7
From 2007.igem.org
Lab schedule
- mouse insulin gene cloning (delay to 8/7)
- primer design for [http://www.informatics.jax.org/searches/accession_report.cgi?id=MGI%3A96573 insulin II of Mus musculus]
- primer for A chain (90-110: GIVDQCCTSICSLYQLENYCN)
- left: GTTTCTTCGAATTCGCGGCCGCTTCTAGATGATTGTAGATCAGTGCTGCAC
- right: GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGTTGCAGTAGTTCTCCAGCT
- primer for B chain
- left: GTTTCTTCGAATTCGCGGCCGCTTCTAGATGTTTGTCAAGCAGCACCTTTG
- right: GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGGACATGGGTGTGTAGAAGA
- primer for A chain (90-110: GIVDQCCTSICSLYQLENYCN)
- primer design for [http://www.informatics.jax.org/searches/accession_report.cgi?id=MGI%3A96573 insulin II of Mus musculus]
- purchase of BCRC human insulin clone (ordered not paid)
- antibiotics LB plate preparation
- ampicillin
- kanamycin
