From 2007.igem.org

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The Plan for the implementation of the blue light system into a functioning BioBrick

• D - Day
• N - Night

Timeline

Week 0 (week beginning 29/07/07)

• ligations ...
• craig starting to make test harness

Week 1 (week beginning 06/08/07)

• D1: Ligate Gen-RBS into LacZ
• D2: Transform onto +Gen,+Kan plates
• D3: Pick colonies and culture
• D4: Miniprep and digest with EcoRI/SpeI. Also digest term (can't remember part name) with EcoRI/XbaI
• N4: Ligate. We might be able to do a short ligation during the day.
• D5: Transform onto +Gen, +Kan plates
• D6: Pick colonies and culture

Transformation into EnvZ-

Week 2 (week beginning 06/08/07)

• D7: Miniprep and digest with XbaI/PstI. Gel purify. Also digest OmpR+ with SpeI/PstI + AP
• N8: Ligate
• D9: Transform onto +Amp plates
• D10: Pick colonies
• D11: Miniprep
• D12: Digest with XbaI/PstI and ligate behind Light receptor plasmid
• D13: Transform into EnvZ-
• D14: Pick colonies

Week 3 (week beginning 13/08/07)

• D1: Make cells competent
• D2: Transform cells with construct.

Week 4 (week beginning 20/08/07)

Week 5 (week beginning 27/08/07)

• Rinse and repeat