From 2007.igem.org
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Composite part of T7 + hemA from CFT073 + hemB
Digest pBca9145-I716179 (EcoR1/BamHI, 2063+1345, S)
Sub into pBca9145-I716157 (EcoR1/BglII, 3082+895, L)
Product is pBca9145-I716181