BerkiGEM2007-AustinConstructionFile57

From 2007.igem.org

Revision as of 01:51, 9 August 2007 by AustinDay (Talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Pre-biobricking modifications to I716094.
Quickchange AD0013F/R to get rid of EcoR1 site.
Quickchange AD0014/F/R to get rid of BglII site.
Quickchange AD0015F/R to get rid of XhoI site.

PCR AD0016F/R on modified I716094.
Digest Bgl2/Xho1 and insert into 9145 plasmid.
Product is I716357

Retrieved from "http://2007.igem.org/wiki/index.php/BerkiGEM2007-AustinConstructionFile57"