NYMU Taipei/Primer List

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Project Description / Lab Notes / Part List / Primer List

Name Sequence Length Tm (C) Restriction site Target length Description
M_INS_A 5' GTTTCTTCGAATTCGCGGCCGCTTCTAGATGGGCATTGTAGATCAGTGCTG 51 bp 56.85 EcoRI, XbaI 119 bp Potential dimer formation!
M_INS_A 3' GTTTCTTCCTGCAGCGGCCGCTACTAGTGTTGCAGTAGTTCTCCAGCT 48 bp 54.74 SpeI, PstI 119 bp Potential dimer formation! Stop codons are removed to enable fusion with export signal.
M_INS_B 5' GTTTCTTCGAATTCGCGGCCGCTTCTAGATGTTTGTCAAGCAGCACCTTTGT 52 bp 60.85 EcoRI, XbaI 149 bp Potential dimer formation!
M_INS_B 3' GTTTCTTCCTGCAGCGGCCGCTACTAGTGGACATGGGTGTGTAGAAGA 48 bp 55.86 SpeI, PstI 149 bp Potential dimer formation! Stop codons are removed to enable fusion with export signal.
H_INS_A 5' GTTTCTTCGAATTCGCGGCCGCTTCTAGATGGGCATTGTGGAACAATGCTG 51 bp 62.45 EcoRI, XbaI 122 bp Potential dimer formation!
H_INS_A 3' GTTTCTTCCTGCAGCGGCCGCTACTAGTGTTGCAGTAGTTCTCCAGCTG 49 bp 57.77 SpeI, PstI 122 bp Potential dimer formation! Stop codons are removed to enable fusion with export signal.
H_INS_B 5' GTTTCTTCGAATTCGCGGCCGCTTCTAGATGTTTGTGAACCAACACCTGTG 51 bp 57.98 EcoRI, XbaI 149 bp Potential dimer formation!
H_INS_B 3' GTTTCTTCCTGCAGCGGCCGCTACTAGTGGTCTTGGGTGTGTAGAAGA 48 bp 55.10 SpeI, PstI 149 bp Stop codons are removed to enable fusion with export signal.
RcsC 5' GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAAATACCTTGCTTCTTTTCG 51 bp 55.92 EcoRI, XbaI 1404 bp
RcsC 3'-2 GGGGTATACCAACGACTCTTCCATCTTCAC 29 bp 59.63 NdeI 1404 bp Three extra guanosine was added to the 5' side of the NdeI cutting site.
[http://partsregistry.org/Part:BBa_G00100 VF2] tgccacctgacgtctaagaa 20 bp 59.44 Forward sequencing primer.
[http://partsregistry.org/Part:BBa_G00101 VR] attaccgcctttgagtgagc 20 bp 59.34 Reverse sequencing primer.
  • [http://www.mdbio.com.tw/pfu.htm pfu primer]
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