NYMU Taipei/Lab Notes/2007 8 24

From 2007.igem.org

Plasmid check

3ul DNA+1ul RE, final volume: 20ul
BBa_R0082 psB1A2_pOmpC
BBa_I15017 EYFP
BBa_I15016 ECFP
BBa_C0076 HSL
BBa_B0034 RBS
BBa_C0077 CinR
BBa_B0014 D-term
BBa_R0077 pCinRHSL RBS+
BBa_R0078 pCinRHSL

NYMU Taipei gel 20070824 plasmid check 1.jpg

  • Well 1 OmpR EcoRI
  • Well 2 OmpR SpeI
  • Well 3 OmpR XbaI, PstI
  • Well 4 EYFP EcoRI
  • Well 5 EYFP SpeI
  • Well 6 EYFP XbaI, PstI
  • Well 7 100bp ladder
  • Well 8 ECFP EcoRI
  • Well 9 ECFP SpeI
  • Well 10 ECFP XbaI, PstI
  • Well 11 HSL EcoRI
  • Well 12 HSL SpeI

NYMU Taipei Gel 20070824 plasmid check 2.jpg

  • Well 1 HSL XbaI, PstI
  • Well 2 CinR EcoRI
  • Well 3 CinR SpeI
  • Well 4 CinR XbaI, PstI
  • Well 5 pCinRHSL EcoRI
  • Well 6 pCinRHSL SpeI
  • Well 7 100bp ladder
  • Well 8 pCinRHSL XbaI, PstI
  • Well 9 Empty
  • Well 10 pCinRHSL+RBS EcoRI
  • Well 11 pCinRHSL+RBS SpeI
  • Well 12 pCinRHSL+RBS XbaI, PstI

NYMU Taipei Gel 20070824 plasmid check 3.JPG

  • Well 1 Empty
  • Well 2 RBS EcoRI
  • Well 3 RBS SpeI
  • Well 4 RBS XP
  • Well 5 100bp ladder
  • Well 6 D-term EcoRI
  • Well 7 Leaky well
  • Well 8 D-term EcoRI
  • Well 9 D-term XbaI, PstI

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