From 2007.igem.org

08/27/07

• New [http://partsregistry.org/Part:BBa_R0051 R0051]+[http://partsregistry.org/Part:BBa_P0412 P0412] ([http://partsregistry.org/Part:BBa_I763010 I763010]) ligation with a new protocol (3h 15°C and 1.2 ul Ligasi).
• Transformation of [http://partsregistry.org/Part:BBa_R0051 R0051]+[http://partsregistry.org/Part:BBa_P0412 P0412] ([http://partsregistry.org/Part:BBa_I763010 I763010]) ligation and of new biobricks [http://partsregistry.org/Part:BBa_S03520 S03520], [http://partsregistry.org/Part:BBa_S0100 S0100], [http://partsregistry.org/Part:BBa_B0034 B0034], [http://partsregistry.org/Part:BBa_J06550 J06550], [http://partsregistry.org/Part:BBa_S0103 S0103].

08/28/07

• We hold a meeting and the topics of the day are:

-model agreed;

-we agree the position of LacI not controlled by Plac but by costitutionaly expressed as PTetR gene (R0040);

-reseach of genomic pLacI sequence to draw the primers or to order the synthesis.

• We transform new biobricks:E0020 (to put after CMV J52034 promoter) and R0040 (whith witch adjust LacI expression);

• We inoculate a S03520, S0100, B0034, J06550, S0103 colony in 5 ml;

• We observe that there isn't a colony to R0051+P0412(I763010) ligation, so we abandon this way.

08/29/07

08/30/07

08/31/07

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