NYMU Taipei/Part Assembly
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Revision as of 10:10, 1 September 2007 by Lihsiangyen (Talk | contribs)
- Enzyme Digestion (2h)
- Restriction enzymes: 1ul each
- Incubate for 2h
- Heat inactivation at 65°C, 20min
- Gel Separation (0.5h)
- 100V, 30min
- Gel Extraction (0.5h)
- Qiagene GelExtraction Kit
- Ligation (3hr)
- T4 ligase: 1ul
- Incubate at RT for 3h
- Plate Culture (16h)
- DNA: 2ul
- Competent cell: 50ul
- Liquid Culture (16h)
- 5ml LB
- Plasmid Extraction (1hr)
- Qiagene Miniprep Kit
- Enzyme digestion to ligation formula
