Alberta/Calender/September
From 2007.igem.org
September
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== September 1 ==
Lab Notes
1. 24 Minipreps for Diezel Blaze in B0034 using Fermentas Kit; stored in -20 freezer
2. Lots of colonies from the transformation last night; no colonies on negation control (good) and lots of colonies on positive control (good) and no satellite colonies (also good); no colonies were picked for overnights
3. Run the Buddy+Benny in B00 and Betty+Enny in B00 Pst/Xba gel from last night at 50V for another 30 minutes for better resolution; all the digest worked so I cut out the appropriate bands and stored the gel in -20 freezer for purification later
-CZ
September 2
September 3
September 4
Schedule:
JP,ML,JG 7:00pm
For the record:
Buddy = BuOH DH 1235 bp
Betty = Bb-bhBu-coa dh 923 bp
Benny = Butyryl coa dh 1184 bp
enny = Enoyl coa Hydratase 860 bp
Diezel Blaze = buAld-Dh 2639 bp
Notes:
Moving was done this mid-day.
New location M349
JG has key & access
- MC, ED, JG
Evening crew - please do a once over of G308 to double check we have not left anything and to clean up anything extra/ things we should get out of the way. thnx - MC
Night Crew
-JP, JG
Set up new lab
Things we need - Water Bath, Scale, disposable 13ml overnight tubes, Tip waste
lab work
Gel purification ran on Betty,enny and boo as well as buddy and benny and is in the -20 in the new lab
To Do list for tomorrow
- Restriction on DB needs to be done - If we want to put it in front of BE or BB then Spe/Pst - If we want to put it behind BE or BB then cut with Xba/Pst - maybe do both (use 4microL DNA for digest)
-complete restriction on BE and BB for SPE and PST (then we can complete ligations BE-BB and BB-BE or we can ligate D.B into BE or BB to make BE-DB or DB-BE or BB-DB or DB-BB- Then we can ligate BE-BB-DB or DB-BE-BB or BE-BB-DB or BB-DB-BE or BB-BE-DB and we are kinda finished!
- If I0500 comes in from calgary it needs to be transformed.
- Transform R0080 which is a back up promoter just in case?
-Note we also have to do sequenceing on BB and BE and DB
Shout out to calgary for saving our necks Shout out to the move in crew to the top
September 5
Schedule:
NG,ED,ML 7:00pm
AM Crew Notes:
finished up cleaning up post-move in
got a 'water bath' - JG calibrated it
prepared BB in Boo & BE in Boo for sequencing - this will be ready for pick up tomorrow
Restricted:
- DB in BOO with PST/XBA
- DB in BOO with PST/SPE
- BB in BOO with PST/SPE
- BE in BOO with PST/SPE
NB: there is a section in the masterbook "figuring stuff out" just a few questions I have from being away so long - don't worry about it we should do a wet lab recap to bring everyone on the same page at our meeting Thrusday - MC
<3 JG & MC
Transformed I0500 from Calgary. Transformed R0080. Ran digestions on gel. Ligated digested DB with BB and BE. (all in Boo)
-ED, ML
September 6
Schedule:
NK ED AL 5:00pm (Unless your crazy enough to show up at 5AM)
To Do:
Ligations
September 7
Schedule:
AM Crew: MC, JG meeting at 800hrs
September 8
Schedule:
NG AL JP Noon?
I'm good to go at 1:15pm -jp
I can be there at 11am - al
September 9
Schedule:
JG ED NG Noon?
September 10
Schedule:
September 11
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