NYMU Taipei/Lab Notes/2007 9 9

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Ligation(H_INS_A)
- H_INS_A1:1ul
- pOmpC:2ul
- 10X ligase:1 ul
- 10X buffer:1 ul
- ddH2O:5ul
- total: 10ul
Transformation
- ligated DNA:2ul
- compenent cell:100ul
apply the cells on the agar plate

plate culture failed!
possibility:concentration of H_INS_A1 is not high enough

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