From 2007.igem.org

Our finally vector will be YCplac22.

This is a YC-type (centromeric) shuttle vector.

Vector Information:

Vector type: plasmid

Size (kb): 4.854

Construct size (kb): 4.854

Construction: pUC19

Markers: TRP1, ampR

Features: insert detection: lacZ'

marker(s): TRP1, ampR

promoter: lac

replicon: ARS1, pMB1

MCS: HindIII...EcoRI

centromere: CEN4

Selected features

lac_promoter 142 - 171

M13_pUC_rev_primer 185 - 207

M13_reverse_primer 206 - 224

M13_forward20_primer 306 - 290

M13_pUC_fwd_primer 321 - 299

CEN4 504 - 1356

Orf frame 2 2740 - 2066

TRP1 2740 - 2066

AmpR_promoter 2983 - 3011

Orf frame 2 3053 - 3913

Ampicillin 3053 - 3913

pBR322_origin 4068 - 4687

Unique restriction sites

HindIII 233

PstI 249

SalI 251

XbaI 257

BamHI 263

SmaI 270

KpnI 276

SacI 282

EcoRI 284

NarI 446

SpeI 550

NheI 1801

BglII 1986

EcoRV 2455

PmlI 2780

AatII 2921