From 2007.igem.org

• PCR issue
  • We tested our PCR product (5uL) from different annealing temperature via GEL
  • From imageJ comparison, the concentration of product rise with temperature before 60 degree
    • GEL (left to right: 49.8, 52.3, 55.5, 58.7, 60.9 degree)
    • 
    • imageJ
    • 
    • marker (100 bp, 40ng/10uL = 4ng/uL)
    • the best temperature should be around 55.5 degree
• PCR product concentration summary

name	product	dulition	A260	A260/A280	A260/A230
9/5 海威	INS_A #1 (A1)	6uL + 594uL ddH2O	0.049	1.27	0.47
9/5 海威	INS_A #2 (A2)	6uL + 594uL ddH2O	0.082	1.32	0.51
9/5 海威	INS_B #1 (B1)	6uL + 594uL ddH2O	0.075	1.29	0.48
9/5 海威	INS_B #2 (B2)	6uL + 594uL ddH2O	0.081	1.36	0.52
9/12 德銘	INS_A #1 (A1, 49.8)	6uL + 594uL ddH2O	0.076	1.33	0.52
9/12 德銘	INS_A #2 (A2, 52.3)	6uL + 594uL ddH2O	0.077	1.33	0.52
9/12 德銘	INS_A #3 (A3, 55.5)	6uL + 594uL ddH2O	0.083	1.29	0.56
9/12 德銘	INS_A #4 (A4, 58.7)	6uL + 594uL ddH2O	0.077	1.33	0.52
9/12 德銘	INS_A #5 (A1, 60.9)	6uL + 594uL ddH2O	0.075	1.33	0.52
9/8 惇茹	INS_A #1	4uL + 196uL ddH2O	0.022	22	n/a
9/8 惇茹	INS_A #1	10uL + 190uL ddH2O	0.056	12	n/a

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• task for the next day
  • GEL preparation for INS_A and INS_B