From 2007.igem.org

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Project Description

The aim is to create a signal generator device that produces an output of PoPS as a function of bacterial cell division. Downstream of the device may be a counter device, quantifiable protein production or some other function of choice.

The system may for example perform pre-determined actions such as programmed cell death after a set number of cell divisions.

The device relies on dif recombinase sites to flip a sequence during each cell division. We hope to further analyse cell division and recombinase mechanisms since bacterial cell division is still relatively poorly understood.

We have constructed and are ligating the bricks required for the first proof of concept experiments.

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Introduction | Applications | Design | Status | References