Biobrick Primers Design
From 2007.igem.org
FOX3 XbaI Fw 5’- GC TCTAGA AGTATGTCGTGCTCGAAGTCA -3’ Lungh=21 Tm 60°C
FOX3 SpeI Rev 5’- GG ACTAGA TGCTTTTATGTAGTGTTAT-3’ Lungh=19 Tm 50°C
LUCIFERASE Xba I Fv 5’- GC TCTAGA ATGGAAGACGCCAAAAACATAA-3’ Lungh=22 Tm 60°C
LUCIFERASE Spe I Rv 5’- GG ACTAGA TTAAAGCTTCTTTCCGCCCTT-3’ Lungh=20 Tm 60°C
2XORE – CYC1 XbaI Fw 5’-GC TCTAGA ATCAGTTATTACCCTCGACCTC-3’ Lungh=22 Tm 58,4°C
1X ORE – CYC1 XbaI Fw 5’- GC TCTAGA GAGATGGCTCGACCATCT-3’ Lungh=18 Tm 56°C
2&1ORE-CYC1 Spe I Rev 5’- GG ACTAGA GCCATGGCCCATCGAATTC-3’ Lungh=18 Tm 56°C