From 2007.igem.org

FOX3 XbaI Fw

5’- GC TCTAGA AGTATGTCGTGCTCGAAGTCA -3’

Lungh=21

Tm 60°C

FOX3 SpeI Rev

5’- GG ACTAGA TGCTTTTATGTAGTGTTAT-3’

Lungh=19

Tm 50°C

Luciferase Xba I Fv

5’- GC TCTAGA ATGGAAGACGCCAAAAACATAA-3’

Lungh=22

Tm 60°C

Luciferase Spe I Rv

5’- GG ACTAGA TTAAAGCTTCTTTCCGCCCTT-3’

Lungh=20

Tm 60°C

2XORE – CYC1 XbaI Fw

5’-GC TCTAGA ATCAGTTATTACCCTCGACCTC-3’

Lungh=22

Tm 58,4°C

1X ORE – CYC1 XbaI Fw

5’- GC TCTAGA GAGATGGCTCGACCATCT-3’

Lungh=18

Tm 56°C

2&1ORE-CYC1 Spe I Rev

5’- GG ACTAGA GCCATGGCCCATCGAATTC-3’

Lungh=18

Tm 56°C