BerkiGEM2007Present1

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Hannah Cole- Alternatives To Hemoglobin:

Over the summer I investigated and created two alternatives to the hemoglobin part in our device. I worked with two genes we believed might allow our blood to carry more oxygen than hemoglobin.

The first gene I will dicuss is H-NOX (Hem-Nitric oxide and Oxygen bonding). H-NOX is a heme-based sensor that is found in bacteria. H-NOX is able to bond to Oxygen using a distal pocket tyrosine. For this gene I added the T7 promoter we created for this project, an RBS site, and lastly a Bca1092 terminator. As I said previously this part would replace the hemoglobin part in our final device. When I assayed this part the results were inconclusive. The part was assembled correctly but the assay didn't show strong signs of expression.

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