From 2007.igem.org

• According to the recommendations made by the members of the computational modeling and Dr. RAUL CUERO we made a last control experiment before running molecular experiments under different environmental conditions from those used at the beginning of the project. Controls were placed under different temperatures, light intensities, and different iron concentrations. The parameters we determined were the same as the rest of the controls (Fe, pH, OD, bacterial growth, DNA, RNA, and protein fluorescence and concentration. All of these were measured at different times).