Biobrick Vector choice

From 2007.igem.org

Revision as of 07:58, 24 September 2007 by Robertina (Talk | contribs)

We have selected from BioBrick plate the pSB1A3 plasmid to clone 1Xore and 2Xore promoters and pho4 coding sequence. This is a high copy number plasmid (copy number of 100-300 per cell) carrying ampicillin resistance. 

                 Psb1A3map.jpg

Instead we have selected J61002 and J61003 plasmids to clone respective Pho80 and Pho8 coding sequence. We have think to insert a promoter element between EcoRI and XbaI and Pho80 or Ph08 Cds with XbaI and SpeI. 

                   Gfp-report2.jpg  Rfp-reporter.jpg
Retrieved from "http://2007.igem.org/wiki/index.php/Biobrick_Vector_choice"