Melbourne/Secondary Reagent Agar Plates

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Contents

Preparation of Agar Plates

Uses

  • Plating out and separating bacterial colonies

Ingedients:


Method:

  1. Add 1.4g of Agar per 100mL of LB
  2. Autoclave (just enough to melt)
  3. Cool down to about 40-50 degrees C on bench/cold room/water.
  4. Add 1/1000 volume of Ampicillin.
  5. Mix and pour into petri dishes. 100ml makes 3-4 plates.
  6. Leave plates to dry on bench/laminar flow partly covered.
  7. Keep in cold room till used

Equipment:

  • Autoclave or Microwave
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