From 2007.igem.org

TATA_INSA, TATB_INSB, TATD_IDE and OmpRBS GEL check after PCR

• from left to right
  • lane 1: 100bp ladder
  • lane 2-4: TATA_INSA (PCR 1+2)
  • lane 5-7: TATB_INSB (PCR 3+4)
  • lane 8-12: OmpRBS (PCR 6)
  • lane 13: 1kb ladder
  • lane 14-16: TATD_IDE (PCR 5+7)
  • lane 17: 100bp ladder
  • lane 18: OmpRBS (PCR 6)

re-culture pOmpC and pCinRHSL

digest TATA_INSA, TATB_INSB, OmpRBS, CinR+HSL+D-term