From 2007.igem.org

Contents 1 GEL check for digestions 1.1 System 1 1.2 System 2 2 three digestion for assembly 3 next tasks

GEL check for digestions

System 1

• TATA_INSA (X.P. digestion, 10/2)
• TATB_INSB (E.S. digestion, 10/2)
• CinR+HSL+D-term (E. digestion, 10/2)

System 2

• OmpRBS (X. P. digestion, 10/2)

• lane1  : 1kd ladder
• lane2-3: TATA_INSA (D. XP)
• lane3-4: TATB_INSB (D. ES)
• lane5-6: OmpRBS (D. XP)
• lane7  : TATD_IDE (D. E)

three digestion for assembly

• check here

next tasks

• pOmpC + TATA_INSA
• TATB_INSB + CinR+HSL+D-term
• pCinRHSL + OmpRBS