Vaibhavi Umesh Notebook
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My Construction Files
My Sequencing Files
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notes
to do
Vaibhaviu 19:02, 1 June 2007 (EDT)
Today we cloned an RFP basic part into pBca9145. So, we did the construction file for pBca9145-Bca1145. We did a PCR of the RFP gene according to the construction file, and the gel is here:
It's a little dilute, but it should work nonetheless. We also digested the vector according to the construction file:
We gel purified the larger of the two bands. It looks like the digest went pretty well, as we see no single-cut plasmid DNA. We took it all the way through to transformation into DH10B cells today.
