pBca1101-Bca1128 [RBS library pool, including pBca1101-Bca1106] (EcoR1/Bgl2, 2942/58/9, Largest) Bca1122 [Cmr] (EcoR1/Bgl2, 2987/1027, Smaller) Ligated product is pBca1101-Bad0002 (Now known as I716052)