Melbourne/Lab BL Notebook/PCR1

From 2007.igem.org

< Melbourne | Lab BL Notebook
Revision as of 14:45, 10 October 2007 by Alisa (Talk | contribs)

=Protocol for PCR reactions A~G= (and -ve control) amplifying the photoreceptor and transmembrane domains of NpSopII-NpHtrII.

PCR mix

  • 5ul 10x buffer
  • 5ul Enhancer
  • 0.6ul dNTPs (25mM stock)
  • 2.5ul MgSO4 (Supplied in PCR kit)
  • 1.5ul Primer I (10uM)
  • 1.5ul Primer II (10uM)
  • 1ul Template (pJS010)
  • 0.4ul Pfx Platinum (Invitrogen)
  • 32.5ul ddH2O

--

  • 50ul Total
Retrieved from "http://2007.igem.org/wiki/index.php/Melbourne/Lab_BL_Notebook/PCR1"