From 2007.igem.org

Protocol for PCR reactions A~G

For amplifying the photoreceptor and transmembrane domains of NpSopII-NpHtrII.

=PCR mix==== ===PCR

5ul 10x buffer\\

5ul Enhancer

0.6ul dNTPs (25mM stock)

2.5ul MgSO4 (Supplied in PCR kit)

1.5ul Primer I (10uM)

1.5ul Primer II (10uM)

1ul Template (pJS010)

0.4ul Pfx Platinum (Invitrogen)

32.5ul ddH₂O

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50ul Total