From 2007.igem.org

Protocol for PCR reactions A~G

For amplifying the photoreceptor and transmembrane domains of NpSopII-NpHtrII.

PCR mix	PCR program
5ul 10x buffer\\ 5ul Enhancer 0.6ul dNTPs (25mM stock) 2.5ul MgSO4 (Supplied in PCR kit) 1.5ul Primer I (10uM) 1.5ul Primer II (10uM) 1ul Template (pJS010) 0.4ul Pfx Platinum (Invitrogen) 32.5ul ddH2O
<math>\sqrt{2}</math>	<math>\sqrt{2}</math>	&radic;2	√2
<math>\sqrt{1-e^2}</math>	<math>\sqrt{1-e^2}</math>	&radic;(1&minus;''e''&sup2;)	√(1−e²)

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PCR mix

|5ul 10x buffer\\

5ul Enhancer

0.6ul dNTPs (25mM stock)

2.5ul MgSO4 (Supplied in PCR kit)

1.5ul Primer I (10uM)

1.5ul Primer II (10uM)

1ul Template (pJS010)

0.4ul Pfx Platinum (Invitrogen)

32.5ul ddH₂O

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50ul Total | |-