Biobrickificreation of pI716101 (Low Copy ampR Plasmid - Produces RFP)
Digest pBca9145-Bca1144#5 (EcoRI/XhoI, 2054+919, S) Sub into pSB4A3-I7101 (EcoRI/XhoI, 3141+1136?, L) Product is pI716101
No Oligos Needed