BerkiGEM2007-DavidConstructionFile103

From 2007.igem.org

Revision as of 06:39, 5 June 2007 by Dtulga (Talk | contribs)
Biobrickificreation of T7rnap I716103, I716104 (T7 RNA Polymerase: ATG, GTG)
---------------------------------------------------
PCR T7rnap-FA and T7rnap-R on pBAC-T7621	(2683 bp, BglII/XhoI, Product = A)
PCR T7rnap-FG and T7rnap-R on pBAC-T7621	(2683 bp, BglII/XhoI, Product = G)
Sub both into pBca9145-Bca1144			(BglII/XhoI, 2063+910, L)
Product A is pBca9145-I716103-T7rnap-A
Product G is pBca9145-I716104-T7rnap-G
---------------------------------------------------
dt005-T7rnap-FA  Forward Cloning of T7rnap ATG Start   ccaaaAGATCTatgaacacgattaacatcgc
dt006-T7rnap-FG  Forward Cloning of T7rnap GTG Start   ccaaaAGATCTgtgaacacgattaacatcgc
dt007-T7rnap-R   Reverse Cloning of T7rnap	       gctagCTCGAGttaGGATCCttacgcgaacgcgaagtccg
Retrieved from "http://2007.igem.org/wiki/index.php/BerkiGEM2007-DavidConstructionFile103"