From 2007.igem.org

Introduction

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Model Parameters

Parameter	Value	Description	Comments		Parameter	Value	Description	Comments
c1max	0.01 [mM/h]	max. transcription rate of constitutive promoter (per gene)	promoter no. J23105; Reference: Estimate		c2max	0.01 [mM/h]	max. transcription rate of luxR-activated promoter (per gene)	Reference: Estimate
lhi	25	high-copy plasmid number	Reference: Estimate		llo	5	low-copy plasmid number	Reference: Estimate
aQ2,R	0.1 - 0.2	basic production of Q2/R-inhibited genes	Reference: Conclusions after discussion		aQ2	0.1 - 0.2	basic production of Q2-inhibited genes	Reference: Conclusions after discussion
aQ1,S	0.1 - 0.2	basic production of Q1/S-inhibited genes	Reference: Conclusions after discussion		aQ1	0.1 - 0.2	basic production of Q1-inhibited genes	Reference: Conclusions after discussion
aQ2,S	0.1 - 0.2	basic production of Q2/S-inhibited genes	Reference: Conclusions after discussion		aQ1,R	0.1 - 0.2	basic production of Q1/R-inhibited genes	Reference: Conclusions after discussion
Degradation constants
dR	2.31e-3 [per sec]	degradation of lacI	Ref. [10]		dS	1e-5 [pro sec]/2.31e-3 [per sec]	degradation of tetR	Ref. [9]/ Ref. [10]
dL	1e-3 - 1e-4 [per sec]	degradation of luxR	Ref: [6]		dQ1	7e-4 [per sec]	degradation of cI	Ref. [7]
dQ2		degradation of p22cII			dYFP	6.3e-3 [per min]	degradation of YFP	suppl. mat. to Ref. [8] corresponding to a half life of 110min
dGFP	6.3e-3 [per min]	degradation of GFP	in analogy to YFP		dRFP	6.3e-3 [per min]	degradation of RFP	in analogy to YFP
dCFP	6.3e-3 [per min]	degradation of CFP	in analogy to YFP		KR	0.1 - 1 [pM]	lacI repressor dissociation constant	Ref. [2]
KIR	1.3 [µM]	IPTG-lacI repressor dissociation constant	Ref. [2]		KS	179 [pM]	tetR repressor dissociation constant	Ref. [1]
KIS	893 [pM]	aTc-tetR repressor dissociation constant	Ref. [1]		KL	55 - 520 [nM]	luxR activator dissociation constant	Ref: [6]
KIL	0.09 - 1 [µM]	AHL-luxR activator dissociation constant	Ref: [6]		KQ1	8 [pM] 50 [nM]	cI repressor dissociation constant	Ref. [12] starting with values of Ref. [6] and using Ref. [3]
KQ2	0.577 [µM]	p22cII repressor dissociation constant	Ref. [11]. Note that they use a protein cII and we have p22cII. Does that match?		nR	1	lacI repressor Hill cooperativity	Ref. [5]
nIR	2	IPTG-lacI repressor Hill cooperativity	Ref. [5]		nS	3	tetR repressor Hill cooperativity	Ref. [3]
nIS	2 (1.5-2.5)	aTc-tetR repressor Hill cooperativity	Ref. [3]		nL	2	luxR activator Hill cooperativity	Ref: [6]
nIL	1	AHL-luxR activator Hill cooperativity	Ref. [3]		nQ1	2	cI repressor Hill cooperativity	Ref. [12]
nQ2	4	p22cII repressor Hill cooperativity	Ref. [11]. Note that they use a protein cII and we have p22cII. Does that match?

Parameter	Value	Description	Comments		Parameter	Value	Description	Comments
c1max	0.01 [mM/h]	max. transcription rate of constitutive promoter (per gene)	promoter no. J23105; Reference: Estimate		c1max	0.01 [mM/h]	max. transcription rate of constitutive promoter (per gene)	promoter no. J23105; Reference: Estimate

Parameter	Value	Description	Comments
c1max	0.01 [mM/h]	max. transcription rate of constitutive promoter (per gene)	promoter no. J23105; Reference: Estimate
c2max	0.01 [mM/h]	max. transcription rate of luxR-activated promoter (per gene)	Reference: Estimate
lhi	25	high-copy plasmid number	Reference: Estimate
llo	5	low-copy plasmid number	Reference: Estimate
aQ2,R	0.1 - 0.2	basic production of Q2/R-inhibited genes	Reference: Conclusions after discussion
aQ2	0.1 - 0.2	basic production of Q2-inhibited genes	Reference: Conclusions after discussion
aQ1,S	0.1 - 0.2	basic production of Q1/S-inhibited genes	Reference: Conclusions after discussion
aQ1	0.1 - 0.2	basic production of Q1-inhibited genes	Reference: Conclusions after discussion
aQ2,S	0.1 - 0.2	basic production of Q2/S-inhibited genes	Reference: Conclusions after discussion
aQ1,R	0.1 - 0.2	basic production of Q1/R-inhibited genes	Reference: Conclusions after discussion
dR	2.31e-3 [per sec]	degradation of lacI	Ref. [10]
dS	1e-5 [pro sec]/2.31e-3 [per sec]	degradation of tetR	Ref. [9]/ Ref. [10]
dL	1e-3 - 1e-4 [per sec]	degradation of luxR	Ref: [6]
dQ1	7e-4 [per sec]	degradation of cI	Ref. [7]
dQ2		degradation of p22cII
dYFP	6.3e-3 [per min]	degradation of YFP	suppl. mat. to Ref. [8] corresponding to a half life of 110min
dGFP	6.3e-3 [per min]	degradation of GFP	in analogy to YFP
dRFP	6.3e-3 [per min]	degradation of RFP	in analogy to YFP
dCFP	6.3e-3 [per min]	degradation of CFP	in analogy to YFP
KR	0.1 - 1 [pM]	lacI repressor dissociation constant	Ref. [2]
KIR	1.3 [µM]	IPTG-lacI repressor dissociation constant	Ref. [2]
KS	179 [pM]	tetR repressor dissociation constant	Ref. [1]
KIS	893 [pM]	aTc-tetR repressor dissociation constant	Ref. [1]
KL	55 - 520 [nM]	luxR activator dissociation constant	Ref: [6]
KIL	0.09 - 1 [µM]	AHL-luxR activator dissociation constant	Ref: [6]
KQ1	8 [pM] 50 [nM]	cI repressor dissociation constant	Ref. [12] starting with values of Ref. [6] and using Ref. [3]
KQ2	0.577 [µM]	p22cII repressor dissociation constant	Ref. [11]. Note that they use a protein cII and we have p22cII. Does that match?
nR	1	lacI repressor Hill cooperativity	Ref. [5]
nIR	2	IPTG-lacI repressor Hill cooperativity	Ref. [5]
nS	3	tetR repressor Hill cooperativity	Ref. [3]
nIS	2 (1.5-2.5)	aTc-tetR repressor Hill cooperativity	Ref. [3]
nL	2	luxR activator Hill cooperativity	Ref: [6]
nIL	1	AHL-luxR activator Hill cooperativity	Ref. [3]
nQ1	2	cI repressor Hill cooperativity	Ref. [12]
nQ2	4	p22cII repressor Hill cooperativity	Ref. [11]. Note that they use a protein cII and we have p22cII. Does that match?

References

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