BerkiGEM2007-KristinConstructionFile1

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Construction of hemA basic part from R. capsulatus
PCR kd001/kd004 on hemA	(639 bp, gp=A)
PCR kd003/kd002 on hemA	(618 bp, gp=B)
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PCR kd001/kd002 on A+B 	        (1237 bp, Bg1II/XhoI)
Digest pBca9145-Bca1144#5  	(BglII/XhoI, 2063+910, L)
Product is pBca9145-I716151
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kd001	Forward Bg1II for hemA	           ccaaaAGATCTatggactacaatctcgcgctc	
kd002	Reverse Xho1 for hemA	           gctagCTCGAGttaGGATCCtcacgcacagcgcgcccagag
kd003	Removing the EcoR1 site in hemA    cgccgatGAGTTCggcgcgc
kd004	Removing the EcoR1 site in hemA    gcgcgccGAACTCatcggcg
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