BerkiGEM2007-KristinConstructionFile4

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Biobricking of hemD
PCR kd011 and kd012 on E.Coli K-12 MG1655	(772bp, Bg1II/XhoI) 
Sub into pBca9145-Bca1144#5  			(BglII/XhoI, 2063+910, L)
Product is pBca9145-I716155
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kd011	Cloning of hemD	ccaaaAGATCTatgagtatccttgtcacccgc
kd012 	Cloning of hemD	gctagCTCGAGttaGGATCCttattgtaatgcccgtaaaag