BerkiGEM2007-DavidConstructionFile105

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Revision as of 04:47, 8 June 2007 by Dtulga (Talk | contribs)
Biobrickificreation of T7 Promoters I716105, I716106, I716107 (Wild Type, Mutant D, Mutant E)
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PCR ca1090F and dt008-PT7-wt			(62 bp, EcoRI/BamHI, Product = A)
PCR ca1090F and dt009-PT7-D			(62 bp, EcoRI/BamHI, Product = D)
PCR ca1090F and dt010-PT7-E			(62 bp, EcoRI/BamHI, Product = E)
Sub each separately into pBca9145-Bca1144	(EcoRI/BamHI, 2063+910, L)
Product A is pBca9145-I716105-PT7-wt
Product D is pBca9145-I716105-PT7-D
Product E is pBca9145-I716105-PT7-E
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dt008-PT7-wt	T7 Promoter (Wild Type)    GtttGGATCCaccTCTCCCTATAGTGAGTCGTATTAtacAGATCTcatGAATTCcagaa
dt009-PT7-D	T7 Promoter (Mutant "D")   GtttGGATCCaccTCTCCCTTTAGTGAGTCGTATTAtacAGATCTcatGAATTCcagaa
dt010-PT7-E	T7 Promoter (Mutant "E")   GtttGGATCCaccTGCTTCTATAGTGAGTCGTATTAtacAGATCTcatGAATTCcagaa
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