BerkiGEM2007-DavidConstructionFile105

From 2007.igem.org

Revision as of 04:49, 8 June 2007 by Dtulga (Talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Biobrickificreation of T7 Promoters I716105, I716106, I716107 (Wild Type, Mutant D, Mutant E)
---------------------------------------------------
PCR ca1090F and dt008-PT7-wt			(62 bp, EcoRI/BamHI, Product = A)
PCR ca1090F and dt009-PT7-D			(62 bp, EcoRI/BamHI, Product = D)
PCR ca1090F and dt010-PT7-E			(62 bp, EcoRI/BamHI, Product = E)
Sub each separately into pBca9145-Bca1144	(EcoRI/BamHI, 2063+910, L)
Product A is pBca9145-I716105-PT7-wt
Product D is pBca9145-I716106-PT7-D
Product E is pBca9145-I716107-PT7-E
---------------------------------------------------
dt008-PT7-wt	T7 Promoter (Wild Type)    GtttGGATCCaccTCTCCCTATAGTGAGTCGTATTAtacAGATCTcatGAATTCcagaa
dt009-PT7-D	T7 Promoter (Mutant "D")   GtttGGATCCaccTCTCCCTTTAGTGAGTCGTATTAtacAGATCTcatGAATTCcagaa
dt010-PT7-E	T7 Promoter (Mutant "E")   GtttGGATCCaccTGCTTCTATAGTGAGTCGTATTAtacAGATCTcatGAATTCcagaa
Retrieved from "http://2007.igem.org/wiki/index.php/BerkiGEM2007-DavidConstructionFile105"