Tokyo/Wash

From 2007.igem.org

Revision as of 08:09, 20 October 2007 by Hiro (Talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Wash


take 200 ml of culture in a Falcon tube into a eppendorf tube (1.6 or 2.0 ml tube)
centrifugation for 2 min at 9000g
discard the supernatant with a pippet
add 200 ml of PBS and dissolve the pellet
centrifugation for 2 min at 9000g
discard the supernatant with a pippet
add 200 ml of PBS and dissolve the pellet
mix well
apply 150 ml onto a well

Retrieved from "http://2007.igem.org/wiki/index.php/Tokyo/Wash"