Boston UniversityStatus
From 2007.igem.org
Contents |
What We've Accomplished
Week's (Ambitious) Goals
Week of 6/4:
1. Evaluate the transformation that was done on Friday.
2. Confirm the correct plasmid (pJQ200)
3. Find appropriate restriction enzymes that cut by BLASTing all the plasmid's restriction enzyme sites onto the global transcription factors.
4. Re-design primers for the global transcription factors based on the restriction enzymes we have selected.
5. Order primers.
6. Practice regular (non-error prone) PCR with the primers to check that they work.
7. Incorporate the global transcription factors into the plasmid and transform this plasmid into the E.coli.
8. Conjugate this plasmid into Shewy.
Materials We Need
Error-Prone PCR: Need to Buy
Restriction Enzymes: bought
Ligases: Need to Buy?
TOPO kit: available in lab
Short-Term To-Do List
Ordering of Error-Prone PCR Materials: Not completed
Thank-You Letters sent to Pfizer: Not Completed
Thank-You Letters sent to BU ppl: Not completed
Protocols
"Calcium Chloride/Heat Shock Plasmid Transformations"
Question and Answer
Can we get the kanR gene with appropriate sticky ends for BsaI and Tth111I? (DB)
Relevant Publications and Links
http://www.shewybase.bu.edu
https://www.atcc.org/common/catalog/numSearch/numResults.cfm?collection=mb-vector&atccNum=77482
