BerkiGEM2007-VaibhaviConstructionFile2

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Revision as of 22:30, 8 June 2007 by Vaibhaviu (Talk | contribs)
Construction of a Biobrick 2.0 basic part in plasmid pBca9145-Bca1144 (Clone # 5) for the SodC open reading frame from Escherichia coli K-12 MG1655

PCR VU01F.sodC/VU02R.sodC on SodC				(553 bp, BglII/XhoI)
 Digest pBca9145-Bca1144 (Clone # 5)		(BglII/XhoI, 2063+910, L)
 Product is pBca9145-VU1
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VU01F.sodC – ccaaaAGATCTatgaaacgttttagtctggc
VU02R.sodC – gctagCTCGAGttaGGATCCttacttaattacaccacaggc
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