BerkiGEM2007-VaibhaviConstructionFile4

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Construction of a Biobrick 2.0 basic part in plasmid pBca9145-Bca1144 (Clone # 5) for the N-terminal methionine aminopeptidase (map) open reading frame from Salmonella typhimurium LT2 

PCR VU07F.map/VU08R.map on methionine aminopeptidase	(817 bp, BglII/XhoI)
Digest pBca9145-Bca1144 (Clone # 5)		(BglII/XhoI, 2063+910, L)
Product is pBca9145-VU3
 -------------------------------------
VU07F.map 	ccaaaAGATCTatggctatctcaatcaagacccc
VU08R.map	gctagCTCGAGttaGGATCCttattcgtcgtgcgagattatcg
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