From 2007.igem.org

An actual demonstration is decided to show the extensible ability of our method.

This demo system

• is designed as simple as possible, without no "cool" logic function;
• includes all the three logic gates, which form into a three-level logic circuit;
• shows that wires can cross and branch off (without interference);
• is loaded on two plasmids, [http://partsregistry.org/Part:BBa_I732998 pSB1A3-I732998] and [http://partsregistry.org/Part:BBa_I732999 pSB1A3-I732999] ,and transformed into Top10 strain in practice;

• accepts aTc and AHL signals as inputs;
• produces RFP and GFP signals as outputs;
• can be "resetted" by IPTG signal (i.e. all outputs lighten when IPTG added in);
• is expected to output the results as the truth table shown in Figure 2.