1. Abstruct

Concept & Model

We made a bacterial society that follows Pareto's principle as an Ant society does.

Requirements

Bistability and coexistence by cell-cell communication.

Genetic circuit

Genetic circuit construction
new parts

GOAL

We indicated our goal of the project by the simulation!! The simulation proved that our genetic circuit have existence of solution.

2. Works

Combinational Wet & Dry Approaches

0. Wet :　Hybrid promoter

1.Dry :　 Formulation

We wrote the Formulation

2.Wet : 　Assay

We decided the parameter of hill coefficients by the assay of AHL & IPTG

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3.Dry : 　Simulation

4.Wet : 　Assay

Simulationを満たすための他のパラメータが現在のプラスミドで可能であるかを、大腸菌を用いた実験で確認した。
1. By the wet experiments using E. coli, we testified whether our current plasmids could satisfy the other required conditions (for the desired behavior of the system) estimated by the simulation.
2. We tested whether our plasmids gave the other parameters to satisfy the simulation by the experiments using E. coli.

5.Future works

Based on the results from the wet experiments in the step 4, the next dry approach - analysis and simulation - should be performed, which will in turn be testified and confirmed by wet experiments. Continueing these processes, if time permits, would further sophisticate our model.

3,About our team

Students
Satoru Akama | Kenichiro Iwasaki | Hiroki Kawahara | Hajime Nakatani

Advisors: Last year's member
Shotaro Ayukawa | Akio Kobayashi

Instructors
[http://www.sb.dis.titech.ac.jp/ Daisuke Kiga] | [http://www.es.dis.titech.ac.jp/ Masayuki Yamamura] | [http://nicosia.is.s.u-tokyo.ac.jp/~hagiya/ Masami Hagiya] | [http://www.sb.dis.titech.ac.jp/ Masahiko Uchiyama]

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