From 2007.igem.org

10/15/07

• O/N ligations for:

-I763019;

-pTet-LacI-T-Plambda-RFP.

10/16/07

• Transformation of I763019 and pTet-LacI-T-Plambda-RFP.
• We inoculate:

-I763005;

-I763015;

-I763004.

• Trasformation of I763019, subsequently growth in 2 mL of LB medium to test if fresh transformed cells have the same problem of vitality seen using the stock.

10/17/07

• Miniprep of:

-I763005;

-I763015;

-I763004.

• We inoculate:

-Ptet-lacI-T-Plambda-RFP-pLac-CI-Lacy-gfp;

-pTet-LacI-T-pLambda-rfp (high copy).

• Control Digestion for results impagination (X/P):

- I763020;

- I763004;

- I763011;

- I763007 (on high copy plasmid);

- I763007 (on low copy plasmid);

- I763033;

- I763015;

- I763012;

- I763005;

- I763031.

10/18/07

• Miniprep:

-Ptet-lacI-T.Plambda.RFP-pLac-CI-Lacy-gfp;

-pTet-LacI-T-pLambda-rfp (high copy).

• Digestion for:

-Ptet-lacI-T.Plambda.RFP-pLac-CI-Lacy-gfp (E/P)

• Band extraction of:

-Ptet-lacI-T.Plambda.RFP-pLac-CI-Lacy-gfp (E/P)

• Ligation for:

-pSB4A3 (E/P)

-Ptet-lacI-T.Plambda.RFP-pLac-CI-Lacy-gfp (E/P)

• Ligation for:

-pLac-GFP(X/P)

- pTetR-LacI (S/P)

• Control Digestion for results impagination:

- I763016 (low copy plasmid);

- I763016 (high copy plasmid);

- I763036;

- I763019;

- I763032;

- I763025 (low copy plasmid);

- I763025 (high copy plasmid);

- I763026;

- I763035.

10/19/07

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