From 2007.igem.org

Contents 1 Towards optical signal processing in E. coli 1.1 Introduction 1.2 Materials and Methods 1.3 Results 1.4 Discussion 1.5 References

Towards optical signal processing in E. coli

iGEM team Freiburg

Introduction

Phytochrome A (PhyA) is a member of the phytochrome family of red/far-red photo-
receptors. These photoreceptors are involved in a wide range of developmental
responses in plants. Fhy1 is a protein which binds PhyA under light conditions,
enable us to use this protein-protein interaction. In plants Fhy1 is responsable
for nuclear accumulation of PhyA in case of light exposure.

Materials and Methods

Cloning of constructs:

PhyA Insert was gained by PCR from plasmid D153ah-phyA using 5´ (5´-TATCGACGAA TTCGCGGCCG CTTCTAGAAA GAGGAGAAAT
TAACTATGTC AGGCTCTAGG CCGAC-3´) and 3´ (5´- AACGATCACT AGTTCCGCCG TGATGGTGAT GGTGGCCTCC GTTATCGAGT TCCACCTCCT TA-3´) primers.
Fhy1 Insert1 was gained by PCR from pGADT7 using 5´ (5´-TATCGACGCT AGCGATTACA TCTATGGGAC TCAGA-3´) and 3´
(5´-AACGATCGGC GCGCCCAGCA TTAGCGTTGA GAAGTAT-3´) primers.
Fhy1 Insert2 was gained by PCR from pGADT7 using 5´ (5´-TATCGACGCT AGCAGTAGTA ATGCTGCTAA GTTTGT-3`) and 3´
(5´-AACGATCGGC GCGCCCAGCA TTAGCGTTGA GAAGTAT-3´) primers.

Results

Discussion

References

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