From 2007.igem.org

Contents 1 Towards optical signal processing in E. coli 1.1 Introduction 1.2 Materials and Methods 1.3 Results 1.4 Discussion 1.5 References

Towards optical signal processing in E. coli

iGEM team Freiburg

Introduction

Phytochrome A (PhyA) is a member of the phytochrome family of red/far-red photoreceptors. These photoreceptors are involved in a wide range of developmental responses in plants. Fhy1 is a protein which binds PhyA under light conditions, enable us to use this protein-protein interaction. In plants Fhy1 is responsable for nuclear accumulation of PhyA in case of light exposure. In our case it wasn´t neccessary to use the full Fhy1 protein, that is why we restriced to the PhyA binding domain of Fhy1.

Materials and Methods

Cloning of constructs:

PhyA Insert was gained by PCR from plasmid D153ah-phyA using 5´ (5´-TATCGACGAA TTCGCGGCCG CTTCTAGAAA GAGGAGAAAT
TAACTATGTC AGGCTCTAGG CCGAC-3´) and 3´ (5´- AACGATCACT AGTTCCGCCG TGATGGTGAT GGTGGCCTCC GTTATCGAGT TCCACCTCCT TA-3´) primers.
Fhy1 Insert1 was gained by PCR from pGADT7 using 5´ (5´-TATCGACGCT AGCGATTACA TCTATGGGAC TCAGA-3´) and 3´
(5´-AACGATCGGC GCGCCCAGCA TTAGCGTTGA GAAGTAT-3´) primers.
Fhy1 Insert2 was gained by PCR from pGADT7 using 5´ (5´-TATCGACGCT AGCAGTAGTA ATGCTGCTAA GTTTGT-3`) and 3´
(5´-AACGATCGGC GCGCCCAGCA TTAGCGTTGA GAAGTAT-3´) primers.

Results

Discussion

References

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