We have a schedule of ligations and transformations that we keep on googledocs to easily access information about the dna and cells in our freezer and fridge. This also organizes what ligations we plan to do in the future. In all there are about 40 ligations we want to do to construct the test constructs and first tristable system and based on the results of the tests, we will need to do as many as 20 more ligations.
