From 2007.igem.org

Protein purification

Equilibration buffer:                                 50 mM Tris×HCl pH=7,5
1 M NaCl
Elution buffer:                                        Equilibration buffer
                                                             + 5 mM Chloramphenicol

Gel filtration buffer:                                50 mM Tris×HCl pH=7,5
                                                            150 mM NaCl