Berkeley LBL/Sonication
From 2007.igem.org
Sonication Purpose: To disrupt cell membranes and release cellular contents.
1. Dissolve cell pellets in TRIS buffer (pH 7.8)
2. Set pulsar to 4 cycles of 15 seconds on mode followed by 10 seconds off mode.
Sonication Purpose: To disrupt cell membranes and release cellular contents.
1. Dissolve cell pellets in TRIS buffer (pH 7.8)
2. Set pulsar to 4 cycles of 15 seconds on mode followed by 10 seconds off mode.
